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ibrd9  (Tocris)


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    Tocris ibrd9
    a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of <t>iBRD9</t> or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.
    Ibrd9, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ibrd9+5591/I-BRD9/pmc11252270-323-12-15
    Average 93 stars, based on 14 article reviews
    ibrd9 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis"

    Article Title: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis

    Journal: Nature Communications

    doi: 10.1038/s41467-024-50225-z

    a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.
    Figure Legend Snippet: a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.

    Techniques Used: Expressing, Control, Western Blot, RNA Sequencing, Staining, Plasmid Preparation, Two Tailed Test

    a The protein expression of STAT1 and CTSK and b STAT1 immunofluorescence (green) in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. c GSEA analysis of DEG profiles in bulk RNA-seq between JQ1 or control vector treated BMCs after RANKL-induction. d Co-IP assay with BRD9 antibody (or IgG) in BMCs during osteoclastic induction, followed by immunoblotting of BRD4 and BRD9. e The protein expression of ARID1A and STAT1 in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. f The protein expression of ARID1A, MMP9 and NFATc1 in JQ1, iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. All experiments were performed in triplicates unless otherwise stated. Source data are provided in the Source data file.
    Figure Legend Snippet: a The protein expression of STAT1 and CTSK and b STAT1 immunofluorescence (green) in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. c GSEA analysis of DEG profiles in bulk RNA-seq between JQ1 or control vector treated BMCs after RANKL-induction. d Co-IP assay with BRD9 antibody (or IgG) in BMCs during osteoclastic induction, followed by immunoblotting of BRD4 and BRD9. e The protein expression of ARID1A and STAT1 in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. f The protein expression of ARID1A, MMP9 and NFATc1 in JQ1, iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. All experiments were performed in triplicates unless otherwise stated. Source data are provided in the Source data file.

    Techniques Used: Expressing, Immunofluorescence, Control, Plasmid Preparation, RNA Sequencing, Co-Immunoprecipitation Assay, Western Blot

    Related Articles

    Mouse Assay:

    Article Title: The bromodomain containing protein BRD-9 orchestrates RAD51–RAD54 complex formation and regulates homologous recombination-mediated repair
    Article Snippet: Each mouse received two injections of a 0.2-ml mixture of 2 million cells with 50% growth factor-reduced MATRIGEL (BD Bioscience). .. Mice bearing tumors of 200 mm 3 were randomly assigned into the indicated groups: vehicle control (saline), PARPi (purchased from Toronto Research Chemicals O514500, 50 mg kg −1 ), iBRD9 (purchased from TOCRIS Cat. No. 5591, 40 mg kg −1 ), and PARPi (50 mg kg − 1 ) together with iBRD9 (40 mg kg −1 ). ..

    Control:

    Article Title: The bromodomain containing protein BRD-9 orchestrates RAD51–RAD54 complex formation and regulates homologous recombination-mediated repair
    Article Snippet: Each mouse received two injections of a 0.2-ml mixture of 2 million cells with 50% growth factor-reduced MATRIGEL (BD Bioscience). .. Mice bearing tumors of 200 mm 3 were randomly assigned into the indicated groups: vehicle control (saline), PARPi (purchased from Toronto Research Chemicals O514500, 50 mg kg −1 ), iBRD9 (purchased from TOCRIS Cat. No. 5591, 40 mg kg −1 ), and PARPi (50 mg kg − 1 ) together with iBRD9 (40 mg kg −1 ). ..

    Saline:

    Article Title: The bromodomain containing protein BRD-9 orchestrates RAD51–RAD54 complex formation and regulates homologous recombination-mediated repair
    Article Snippet: Each mouse received two injections of a 0.2-ml mixture of 2 million cells with 50% growth factor-reduced MATRIGEL (BD Bioscience). .. Mice bearing tumors of 200 mm 3 were randomly assigned into the indicated groups: vehicle control (saline), PARPi (purchased from Toronto Research Chemicals O514500, 50 mg kg −1 ), iBRD9 (purchased from TOCRIS Cat. No. 5591, 40 mg kg −1 ), and PARPi (50 mg kg − 1 ) together with iBRD9 (40 mg kg −1 ). ..



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    ibrd9  (Tocris)
    93
    Tocris ibrd9
    a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of <t>iBRD9</t> or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.
    Ibrd9, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ibrd9+5591/I-BRD9/pmc11252270-323-12-15
    Average 93 stars, based on 1 article reviews
    ibrd9 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.

    Journal: Nature Communications

    Article Title: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis

    doi: 10.1038/s41467-024-50225-z

    Figure Lengend Snippet: a The protein expression of ARID1A and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. b GSEA analysis of DEG profiles in bulk RNA-seq between the control group and LysM-Cre;Arid1a fl/f group. c The mRNA expression of Stat1 and Ifnb1 in BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. d TRAP staining and quantification analysis of iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. Scale bar, 100 μm. n = 5 biologically independent samples. e The mRNA expression of Mmp9, Acp5 and Dcstamp and in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. n = 4 biologically independent samples for Mmp9 and Acp5 . n = 3 biologically independent samples in Dcstamp . f The protein expression of ARID1A, MMP9, and CTSK in iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction, as measured by western blot. All data in this figure are represented as mean ± SD. Two-tailed Student’s t -test for c . One-way ANOVA with Tukey’s multiple comparisons test for d and e . All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.

    Article Snippet: For the inhibitor experiments, the cells were treated with either vehicle or iBRD9 (1 μM, Tocris Bioscience, 5591), JQ1 (0.08 μM, Abmole Bioscience, M2167), and SP-141 (0.06 μM, Csnpharm, 223123) twenty-four hours after plated.

    Techniques: Expressing, Control, Western Blot, RNA Sequencing, Staining, Plasmid Preparation, Two Tailed Test

    a The protein expression of STAT1 and CTSK and b STAT1 immunofluorescence (green) in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. c GSEA analysis of DEG profiles in bulk RNA-seq between JQ1 or control vector treated BMCs after RANKL-induction. d Co-IP assay with BRD9 antibody (or IgG) in BMCs during osteoclastic induction, followed by immunoblotting of BRD4 and BRD9. e The protein expression of ARID1A and STAT1 in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. f The protein expression of ARID1A, MMP9 and NFATc1 in JQ1, iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. All experiments were performed in triplicates unless otherwise stated. Source data are provided in the Source data file.

    Journal: Nature Communications

    Article Title: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis

    doi: 10.1038/s41467-024-50225-z

    Figure Lengend Snippet: a The protein expression of STAT1 and CTSK and b STAT1 immunofluorescence (green) in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. c GSEA analysis of DEG profiles in bulk RNA-seq between JQ1 or control vector treated BMCs after RANKL-induction. d Co-IP assay with BRD9 antibody (or IgG) in BMCs during osteoclastic induction, followed by immunoblotting of BRD4 and BRD9. e The protein expression of ARID1A and STAT1 in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. f The protein expression of ARID1A, MMP9 and NFATc1 in JQ1, iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1a fl/fl mice and littermate control mice after RANKL-induction. All experiments were performed in triplicates unless otherwise stated. Source data are provided in the Source data file.

    Article Snippet: For the inhibitor experiments, the cells were treated with either vehicle or iBRD9 (1 μM, Tocris Bioscience, 5591), JQ1 (0.08 μM, Abmole Bioscience, M2167), and SP-141 (0.06 μM, Csnpharm, 223123) twenty-four hours after plated.

    Techniques: Expressing, Immunofluorescence, Control, Plasmid Preparation, RNA Sequencing, Co-Immunoprecipitation Assay, Western Blot